qPCR is actually did to decide gene backup number to own germs and you may fungi, including ?-Proteobacteria, ?-Proteobacteria, Fimiricutes, Actinobacteria, Acidobacteria and you may Basidiomycota, with the primers and you can conditions demonstrated in past times because of the Trivedi ainsi que al. (2012). Spearman relationship analyses was in fact did using the XLSTAT application (Addinsoft SARL, Paris, France) to evaluate the partnership ranging from relative variety determined by qPCR and you may the relative abundance dependent on pyrosequencing.
Mathematical and you may analytical analyses
We first searched if or not enough spatial variability is obtained inside our crushed products in terms of microbial area construction and you can capabilities (creation of enzymes involved in C degradation) to check on our very own hypothesis.